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To ensure that we actually worked with strength and aerobic exercises, we used exercises that are generally used in our gold standard maximal strength tests and aerobic tests (the additional running is also used very often in VO2max tests; see, e.g., [26]. For the protocols, we limited this to 70% of the maximum and ensured that the duration (including rest periods) was similar for both exercises.

At least two weeks before the infusion protocols, maximal strength tests were determined for each leg as a one repetition maximum (1RM; i.e., the maximal amount of weight lifted for one repetition during a concentric contraction, using a standard leg extension machine (Badger 2001 series by Magnum Fitness Systems, South Milwaukee, WI, USA).

All volunteers were evaluated by a medical doctor and were considered to be able to perform maximal strength tests.

Average power per training session, maximal strength tests (maximal exercise repetitions/min), three performance-based tests for physical function, SF36 for QoL were assessed in the beginning and every 6 months thereafter.

When combined, tests such as rowing performance (Baguet et al. 2010a), running performance (Derave et al. 2007; Kern and Robinson 2011) and maximal strength tests (Kendrick et al. 2008) had an effect size of 0.150 −0.0599 to 0.473) in the Pla group and 0.326 (0.164 0.549) in the BA group, although this was not significantly different (P = 0.204; Fig. 1).

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Reliability of maximal strength testing in older adults.

Participants will perform a 5 min warm-up on a bicycle ergometer before maximal strength testing.

On the following visit, the first maximal strength test session was performed.

After 72 hours, a second maximal strength test was performed and the value obtained was used in this study, only for strength assessment.

For safety reasons, exclusion criteria (pregnancy, diagnosed angina pectoris, life-threatening diseases) were introduced since maximal strength testing was part of the evaluation.

Our observations are consistent with those of Gordon et al who reviewed the safety of dynamic maximal strength testing in older adults and did not identify a single cardiovascular event in over 26,000 assessment sessions.

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