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Samples were centrifuged at maximal speed for 10 min, yielding the proteins in the supernatant.
Participants were asked to walk barefoot without any assistive devices at their self-selected normal and maximal speed for 5 trials.
PCMOs were washed with PBS, centrifuged and diluted with PBS containing 1% BSA, centrifuged at maximal speed for 3 min using the Cytospin 4 centrifuge (Thermo Scientific) and kept in −20°C until needed.
Mice from the Exe and Exe + LPS groups were then trained using low intensity exercise (50% of the mean maximal speed) for 60 minutes/day, 3 days/week, for 5 weeks [ 3].
Sample were pelleted at maximal speed for 10 min at room temperature and the supernatant was separated by SDS-PAGE and the fluorescence was detected and quantified in the gel using the BioRad ChemiDoc MP Imaging System (BioRad, Hercules, California, USA).
Similar(55)
The endurance test was performed on 2 h fasted weight-matched E2f1 +/+, −/−, +/+; mdx and −/−; mdx mice at the beginning speed of 10 cm/s for 5 min. The speed was progressively increased (around 2 cm/s each 5 min) to reach, at the end of the running test, the maximal speed of 25 cm/s.
Cervical motor control is assessed in a test of maximal speed of cervical axial rotations [ 32].
Because of its quantitative precision, specificity, and compatibility with high throughput robotic operations, the LOA assay eliminates bottlenecks in ES cell screening and mouse genotyping and facilitates maximal speed and throughput for knockout mouse production.
Cells were vortexed for 10 seconds to ensure thorough lysis before nuclei were collected by centrifugation at maximal speed in a microfuge for 5 minutes at 4°C.
Average critical swimming speed (the maximal speed animals can swim with for extended periods of time) for males of this population of guppies is 13cm/s (Lievens 2012).
When the tasks were performed at maximal speed, older participants were faster for only one task.
Related(20)
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