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Of these, C.vulgaris ESP-31 had the best tolerance towards wastewater, with maximal biomass concentration of 2.82 g/l and lipid productivity of 32.3 mg/l/d.
Then the growth of C. acetobutylicum started and reached a µmax of 0.21 h−1 and maximal biomass concentration of 3.7 g/L after 12 h of culture.
The main effects and interactions of three factors (agitation, aeration and copper induction) over five responses (μ, αLacc, βLacc, maximal volumetric laccase activity and maximal biomass concentration) were analyzed.
The maximal OD600 values reflecting the maximal biomass concentration with 50 mM D-galacturonate consequently were lower (OD600 of about 6.5) than with 50 mM glucose (OD600 of about 18).
In addition, the PB11 pykAF− ppsA− strain had a 29% lower maximal biomass concentration and YX/Glc+Ace compared with its parental strain, PB11 (Table 2004).
All indicated strains displayed the growth kinetics under fed-batch fermentation process at DO-stat 30% in glucose minimal medium very similar to that of the wild-type strain, except slightly elevated maximal biomass concentration of mcGSH2 and mcMET4 recombinant strains (by 8%and13%3%, respectively).
Similar(54)
The mixotrophic cultivation results in faster growth and increased maximal biomass concentrations compared to photoautotrophic cultures [ 64– 64].
Maximal cell biomass concentration was higher under conditions of high saturation with oxygen (shake agitation at 600-800 rpm) as compared to low aeration (200 rpm), independently on the mode of pH control.
In turn, minimal biomass concentration was obtained in run no.
Although specific activity requires moderate concentration of glucose and relative high pH, maximal biomass was achieved in the presence of a relative high concentration of glucose and low pH.
The kind and concentration of available growth substrates influence a cell's maximal biomass yield [ 23- 26].
Related(17)
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