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It is recognised that the gene expression correlation method failed to predict glycerol transport at the 75% maximal biomass level, and underestimates this at the 85% maximal biomass level.
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After obtaining the maximal biomass production flux by linear programming, we further maximized the production flux of target metabolites under fixed biomass production flux on the maximal value to avoid alternative production flux.
Both maximal biomass growth (5.17 g/l) and lipid productivity (68.4 mg/l/d) was significantly enhanced with activated charcoal addition.
Inorganic nitrogen in the form of nitrate NO−3 instead of ammonium (NH4+) was required for maximal biomass production.
The main effects and interactions of three factors (agitation, aeration and copper induction) over five responses (μ, αLacc, βLacc, maximal volumetric laccase activity and maximal biomass concentration) were analyzed.
In terms of their maximal biomass glucose, maltose-, and galactose-limited cultures were comparable and slightly below the glycerol-limited culture (which achieved the highest biomass), while lactate-limited growth resulted in about one half of that level.
Biopolymer production was maximal at 25°C, while maximal biomass was obtained at 30°C.
To retain the detected optima (e.g., maximal biomass), the biomass flux was fixed at the optimal value during minimization.
The early induction led to the lowest growth rate and lowest maximal biomass.
FBA returns a single flux distribution that corresponds to maximal biomass production under given growth conditions.
For example, one may compute the maximal biomass production rate under some specific growth conditions.
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