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Briefly, I study the interaction between cells and the extracellular matrix in bone.
Osteopontin (OPN) is an important component of the extracellular matrix in bone.
This also correlates with the histological findings of an absence of collagen (the main component of the organic matrix in bone and dentin) in highly mineralized areas.
It was generally considered that ordered proliferation and differentiation of osteoblast was indispensable for mineralization of extracellular matrix in bone formation during wound healing [ 6].
The alteration of the organic matrix in bone by advanced glycation end products could be relevant to increased bone fragility with aging and in such disease as diabetes (19, 20).
The organic matrix in bone consists of approximately 90% type I collagen, while the remaining 10% is composed of proteoglycans and numerous non-collagenous proteins, of which osteocalcin and osteonectin constitute 40%to50%0% [ 22, 23].
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We synthesized a novel porous scaffold, a collagen sponge incorporating a hydroxyapatite/chondroitinsulfate composite (pCol-HAp/ChS), containing materials which resemble extracellular matrices in bone and cartilage tissues, which needs high compressive strength for clinical use.
These polymeric materials (PLA, PGA and PLGA) have been used as temporary extracellular matrices in bone tissue-engineering scaffolds [ 64] as well as sutures, thereby providing evidence of safety [ 27].
We used an artificial matrix containing laminin and Collagen I (the predominant matrix molecules in bone) and we showed that the parental and the metastatic cells had similar ability to migrate across the membrane (Fig 1 C D).
Though there are many bone matrix components, this review focuses on collagen, the most abundant matrix protein in bone and a determinant of bone strength and quality [ 13].
This versatile, fibre-forming technique is ideal as the resulting submicrometre fibres mimic the structure of the natural extracellular matrix found in bone.
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