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Appropriate cells are seeded to the matrix in vitro.
Achieving substantial cellular infiltration within the electrospun matrix in vitro remains time consuming and challenging.
Formation of hydroxyapatite crystals on the matrix in vitro has been confirmed by FTIR, XRD and SEM-EDAX techniques.
We are investigating the generation of osteoinductive matrix in vitro by culturing cell/scaffold constructs for tissue engineering applications.
The potential of the metal-free hydrogel as a cell scaffold was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) within the gel matrix in vitro.
The potential of the composite hydrogel as an injectable scaffold was demonstrated by the encapsulation of bovine articular chondrocytes within the composite hydrogel matrix in vitro.
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Investigating important genes of cartilage turnover, with respect to chondrocytes cultivated in a 3 D matrix in-vitro, we found a more universal up-regulation rather than a specific one.
Regarding individual gene expression, in vivo regenerate cell-matrix properties were significantly dependent on initial cell-matrix in vitro values as a sign of linearity.
A viability assay suggested biocompatibility of these matrices in vitro.
Aligned nanofibers significantly enhanced cell infiltration into the nanofibrous matrices in vitro.
The established methodology is concluded to provide generally applicable new options for tailoring tissue-specific multiphasic matrices in vitro.
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