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D) Hit ratio: Number of peptide masses matched/number of masses submitted to the peptide mass fingerprint database search.
Peptide matching and protein identification were performed using Mascot peptide mass fingerprint database (Matrix Science Ltd ,UK) following standard search parameters.
MALDI-TOF/TOF determined the identity of HOXC11 protein and detected peaks were matched against Mascot peptide mass fingerprint database for homology search (Additional file 1).
For the database search, listed contamination peaks from keratin and autoproteolytic products were excluded for peptide mass fingerprint database search with the Mascot server (http://www.matrixscience.com) in the NCBInr database.
Using carbon tetrachloride (CCl4) as a model for toxic liver injury in mice, and Abcb4-knockout mice as a model for sclerosing cholangitis to study differentially expressed proteins in liver tissue, Henkel et al. combined DIGE with MALDI-TOF MS and peptide mass fingerprint database search.
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In-gel digestion, sample preparation, MALDI TOF and MALDI TOF-TOF peptide mass fingerprint and database searching was carried out as described in detail elsewhere [ 46].
In peptide mass fingerprint (PMF) map database searching, Mascot Distiller software was used to determine the monoisotopic peak list from the raw mass spectrometry files.
There are publicly available search engines that can match the mass fingerprint against this database for over 1,000 different types of organisms.
The masses of the tryptic fragments obtained from MALTI-TOF/MS were compared to the predicted p53 tryptic peptide map by peptide mass fingerprinting using database-matching procedure.
We analyzed the data using the Mascot program (http://matrixscience.com) [16] to match the peptide mass fingerprint to two databases, NCBI and MSDB.
The resulting peptide spectra were interpreted with Micromass proteinprobe software and analyzed with MASCOT peptide mass fingerprint software and databases (both available at http://www.matrixscience.com).com
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