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A method was established to enrich for preferred TG2 substrates in a complex gluten peptide mixture by tagging with 5-biotinamido-pentylamine. Tagged peptides were isolated and then identified by nano-liquid chromatography online-coupled to tandem mass spectrometry, database searching and final manual data validation.
The endogenous metabolites in the urine were identified using the NIST 2008 mass spectrometry database.
After trypsin digestion and analysis of the digested peptides by mass spectrometry, database searches enable protein identification.
Conclusions:High-throughput computational analysis can provide a viable second stage validation of primary mass spectrometry database search results.
Here, we introduce RiPPquest, a tandem mass spectrometry database search tool for identification of microbial RiPPs, and apply it to lanthipeptide discovery.
The tandem mass spectrometry data acquisition enabled automatic search and matching against the METLIN tandem mass spectrometry database, shortening the current workflow from days to hours.
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The assays identified 58 potential ERK-primed GSK-3 substrates, of which 23 had evidence for in vivo phosphorylation in mass spectrometry databases.
Often microarray chips or mass spectrometry databases are designed using the best available sequence from the research strain, yet then use RNA and protein samples from another similar virulent strain.
However, the likelihood that there is a protein encoded on either strand of the TERC locus is extremely remote, as indicated by codon usage/substitution patterns in the ORFs, the lack of peptides in mass spectrometry databases that map to these ORFS, and the lack of homology of any of the ORFS to any known protein sequence.
These formats are compatible with common mass spectrometry databases and software packages.
Peptides coded by subtelomeric genes (trans-sialidases, RHS, DGF-1, RNA helicases and N-acetyltransferase) were detected in mass spectrometry databases using the gene id number.
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