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BMSCs were extracted from the 4-week-old male rats by the method of bone marrow culture [50].
Autologous BMSFs in AML were isolated from the stromal layers of long-term bone marrow culture (LTBMC) using immunomagnetic beads.
The research makes extensive use of techniques such as in vitro bone marrow culture systems, flow cytometry, polymerase chain reactions, microarray analysis, and animal surgery, not all of which Sukhai is able to do.
The present study was designed to elucidate the mode of action of isoproterenol (Isp; adrenergic β-agonist) and to characterize the effect of the calcitonin gene-related peptide (CGRP; sensory neuropeptide) on osteoclast formation induced by Isp in a mouse bone marrow culture system.
The present studies were designed to investigate whether similar ontogenic differences exist in the expression of cytokine in stromal cells from FBM, ABM, and CB using RI-PCR, and how the differences affect the repopulating ability of the hematopoietic stem cells after long term bone marrow culture (LTBMC).
All patients with positive blood or bone marrow culture for S. typhi and S. paratyphi A (per protocol analysis) and separately all randomised patients (intention to treat analysis) were analysed.
Similar(18)
For non-adherent bone marrow cultures, cells were first cultured in 75 cm tissue culture flasks (15×10 cells per flask) with M-CSF (25 ng/ml).
For total bone marrow cultures, cells were plated at a density of 2.5×10 cells/cm and cultured in MEM supplemented with 1% penicillin streptomycin and 10% FBS in the presence of RANKL (50 ng/ml) and AA (50 µg/ml).
The present studies were designed to determine whether reduced mitochondrial generation of toxic radical oxygen species in NOS1−/− mice also increased the longevity of hematopoiesis in continuous bone marrow cultures and conferred radioresistance to cells in vitro.
In some patients bone marrow cultures were obtained.
Blood or bone marrow cultures were only obtained if clinical symptoms were indicative of acute infection.
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