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Toluidine staining allows observation and identification/discrimination of tissues (e.g. bone and bone marrow) around the metal implant interface without removing the PMMA before the histological examination in a light microscope (Nikon Eclipse Ti, Tokyo, Japan).
Finally, hematopoietic cells move into the bone marrow around the perinatal stage to constitute the adult-type hematopoietic system.
In mice, hematopoiesis transitions from the liver to the spleen in the older fetus, and then to the bone marrow around the time of birth.
In our rat model the foreign body was implanted as a knee prosthesis, to mimic the human condition in which the osteomyelitis process is initiated in the bone marrow around the prosthesis.
Even though we observed GFP+ pericytes in the bone marrow around the blood vessels, albeit at low frequency, we did not find a significant increase in GFP+ cells in response to stroke.
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Total bone marrow (around 5 ml) was sampled after the last boost to isolate RNA using Tri Reagent (Molecular Research Center Inc., Cincinnati, USA) according to the manufacturer's instructions.
Most MRI 'erosions' appearing to be larger in volume than corresponding lesions detected by CT may occur because MRI depicts abnormalities in bone marrow around, as well as filling, bone defects [ 23].
In the slides prepared according to technique 2, only well-spread marrow cells around the bone marrow particles were analysed.
This latter is defined as the fat internally stored in the bone marrow, and around the main organs such as heart, lungs, liver, spleen, kidneys, intestine, muscles, and the lipids rich tissues surrounding the central nervous system (Heymsfield et al., 1997).
After 24 months treatment with infliximab, T1-weighted images showed a decrease of signs of acute inflammation such as bone marrow edema and maybe even an increase of post-inflammatory fatty bone marrow degeneration around the disk margins.
Minorities are under-represented in bone marrow registries around the world, including among the 9 million volunteers registered with the United States National Marrow Donor Program.
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