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Exact(6)

CAPS markers were resolved on silver stained polyacrylamide gels [ 85].

Specifically, 766 SNP markers were resolved into 23 LGs (Table 1; Figure 3).

Disagreements about the markers were resolved and consensuses were reached after discussion.

Markers were resolved into nine autosomal and a Z-chromosome-specific linkage group, 10 markers remaining unlinked.

The microsatellite markers were resolved on the LI-COR system and data scored as described in Rajora et al. [ 42, 43].

The cycling parameters were as follows: 94°C for 3 min; 30 cycles of 95°C for 30 s, 58°C for 45 s and 72°C for 3 min; 72°C for 3 min. Amplicons of mPing-SCAR markers were resolved in 1.0% agarose gels, stained with ethidium bromide and visualized under UV light.

Similar(54)

A subset of polymorphic markers was resolved on ABI3100 DNA Genetic Analyzer and analyzed using GeneMapper v 3.7 (Applied Biosystems).

The poorly resolved region in the male genetic map of LG22 (shown by the red line at 33 cM, nine out of 30 markers) was resolved into 170 cR, which corresponds to 11.4 Mb.

Prior to digestion, a 10  μL aliquot of each PCR product, together with the 100 bp ladder (Biogene) which acts as a reference marker, was resolved by gel electrophoresis on 1.5% resolution agarose gel for 40 min at 70 V.

We mapped 809 AFLP markers that were resolved by 40 primer combination, showing an average multiplex ratio of 20 markers per primer combination.

The female and male parental maps contained 235 and 280 markers and were resolved into 43 linkage groups covering 1604 cM and 1496 cM, respectively.

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