Sentence examples for markers were optimized from inspiring English sources

Primers were designed and microsatellite markers were optimized.

Microsatellite markers were optimized for the allelic range and multiplexed.

Primers for the 12 new microsatellite markers were optimized using Primer3 (v.0.4.0) according to the bovine genome sequence data currently available (i.e. Baylor release Btau_4.0, http://genome.ucsc.edu/cgi-bin/hgGateway) and the appropriate fragment size in the currently designed marker set.

The aggregate prognostic markers were optimized to predict progression in the KL score.

PCR primers for selected differentially expressed genes and gene expression markers were optimized using parental genomic DNA with Annealing Temperature Gradient PCR (ATG-PCR; Additional file 1: Table S5).

PCR cycling conditions for all Type I markers were optimized for P. maniculatus and P. polionotus DNA using a MJ Research PTC-200 DNA Engine gradient thermal cycler and are defined as follows: 1) Standard: 95°C for 14.5 min followed by 35 cycles of (95°C for 30 s, 48 65°C for 30 s, 72°C for 30 s per 0.5 kb), 72°C for 10 min, 4°C hold.

For each genetic marker the sequences obtained were optimized by eye in BIOEDIT [40] in alignment with the orthologous sequences described in the previous section.

Genotyping with SSR markers: The PCR conditions for SSR amplification were optimized for each primer pair.

Vascular markers for vascular smooth muscle cells, endothelial cells and collagen IV were optimized using the avidin-biotin complex (ABC) detection method.

They were optimized for ramming.

Fermentation conditions were optimized.