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Differences of more than two nucleotides were chosen to design InDel markers and single nucleotide polymorphisms were used to design SNP markers.
To construct a high-density linkage map for fine mapping in the target region, new insertion/deletion (InDel) markers and single nucleotide polymorphism (SNP) markers were developed according to the sequence differences between indica var.
The deletion was confirmed to be of maternal origin, by microsatellite markers and single nucleotide polymorphisms (SNPs) analysis, as shown in Fig 1B.
Assignment of Y-HG and subclade in this study used the tests for the markers and single nucleotide polymorphisms (SNPs) listed in Table S1 (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/651415).org/10.1155/2015/651415
However, for effective gene discovery and marker-assisted breeding, it is important to develop more user-friendly, efficient, transportable and co-dominant markers such as simple sequence repeat (SSR) markers and single nulceotide polymorphism markers (SNPs).
This study aimed to unravel complex structures of the Hf region on chromosome 1AS in wheat by using genotyping-by-sequencing (GBS) markers and single nucleotide polymorphism (SNP) markers.
Similar(50)
To further refine the locus, we used autozygosity mapping, genotyping over 200 microsatellite markers and single-nucleotide polymorphisms (SNPs) within the published locus in two affected individuals (Table S1, available online).
We present here such an approach, relying on facilitated recovery after spontaneous excision – or 'popouts' – of dominant transgenes stably inserted into the ribosomal RNA array, utilizing GFP as a marker and single cell sorting to recover regenerated WT controls.
The system consists of a camera, eight LED markers, and a single board computer mounted to each shoe.
A high-resolution genetic and physical map for the fine mapping of NUDA was constructed by the analysis of 11 newly developed markers, including 10 insertion/deletion (InDel) markers and one single nucleotide polymorphism (SNP) marker in 1,192 F2 Nuda individuals derived from the TN1 and HMK cross (Table1).
The model assumes selective neutrality of unlinked markers and a single, closed population.
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