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There are several different types of genetic sequence variants, including single nucleotide polymorphisms (SNPs), small deletion and insertion polymorphisms (indels), micro-satellite markers, and polymorphic insertion elements such as retrotransposons [ 6].
Information of molecular markers and polymorphic summary among DPRK accessions.
Next, we performed the enrichment analysis for each type of SSR in the confirmed markers and polymorphic markers, respectively.
Of the markers, the highest number of total markers and polymorphic markers per locus were produced by SRAP, followed by SSR and CAP-SNP markers.
In a recent simulation study, where markers were compared for mapping accuracy, a difference in power between bi-allelic markers and polymorphic markers was reported, with a higher density of SNPs required to accurately produce similar results to the polymorphic microsatellites [ 32].
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This technique can be used to determine the order and distance between DNA markers by analyzing their presence or absence in hybrid cells, and to localize both polymorphic markers and non-polymorphic markers, such as ESTs.
In the present study, the parents, IR20 and NP, were genotyped with 635 rice microsatellite markers, and 25 polymorphic markers were used in genotyping the mapping progenies.
Two newly developed markers and two polymorphic SSR markers (RM17713 and RM13) were used to construct a linkage map of NUDA/GL-1 using Mapmaker/Exp 3.0 (Lander et al[1987]) on the basis of the 158-plant F2 population.
Among the 1,576 reported markers and 1,319 polymorphic DArT markers, those with P value < 0.05 were used in the current study.
To overcome this limitation, the Illumina (1M) and Affymetrix arrays (Affymetrix 5.0 and 6.0) include more SNP markers and non-polymorphic probes to cover CNV-rich regions.
Eight ISSR primers generated a total of 96 polymorphic markers and revealed high polymorphism among the studied species.
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