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16S rRNA gene profiling involves targeted amplification and subsequent next-generation sequencing of phylogenetically informative marker sequences of microbial DNA [ 20].
We subsequently explored the feasibility of altering the prototrophic marker sequences of other commonly used pRS plasmids to both increase the availability of unique sites in their polylinkers as well as to standardize unique site selection across the series.
This is due to the presence of several restriction sites within the S. cerevisiae HIS3, TRP1, LEU2, URA3 and other yeast-selectable marker sequences of the pRS plasmids that are also found in the pBluescript/pBluescript II MCS of the pRS plasmid backbone (Brachmann et al. 1998; Eriksson et al. 2004; Sikorski and Hieter 1989).
For each marker, sequences of 53 C. hominis specimens were used for calculation of haplotype diversity (Hd), number of haplotypes, number of polymorphic and segregating sites, intragenic linkage disequilibrium (LD), and intragenic recombination rates by using DnaSP version 5.10.00 (www.ub.es/dnasp/).ub.es/dnasp/
The 1,433 yellowtail EST marker sequences of the RH map were compared with the cDNA sequences of four fish species: medaka (Oryzias latipes), zebrafish (Danio rerio), three-spined stickleback (Gasterosteus aculeatus) and green-spotted pufferfish (Tetraodon nigroviridis) using the TBLASTX algorithm.
Similar(55)
Interestingly, the marker sequence of one of the var.
What the Cornell/Stanford team has done is to produce genomic maps of more than 1,000 samples, which link the presence of genetic "markers" (sequences of DNA) to traits such as acidity, sugar content, or disease resistance.
Considering the similarity of marker sequences to reference BESs and BACs, all markers except 074I10_3_2 were considered to be specific labels of appropriate BAC clones.
Initial marker order was predicted based on alignment of DG marker sequences to the reference genome sequence [ 18].
For all markers, sequencing of products confirmed that HaeIII or TaqI restriction sites occurred at the appropriate positions to result in the restriction fragment size polymorphisms that were used to assign markers to chromosomes (Table 2).
Meanwhile, by aligning the marker sequence to the "Darmor" genome of B. napus [ 23], an additional 156 marker sequence could be assigned to certain ancestral blocks.
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