The locus sequences of DNA linear Bin A and Bin B binary toxin genes, partial cds were deposited in the GenBank under the accession numbers JN007909, JN0079010, and JN0079011, JN0079012 for B. sphaericus EMCC 1931 and EMCC 1932, respectively.
For other gene loci, prototype sequences needed for the design of MLST were obtained from the genomes of L. interrogans serovar Lai (NC_004342 and NC_004343) and L. interrogans serovar Copenhageni (NC_005823 and NC_005824) The multi locus sequences of all the 271 isolates obtained as a part of this study are available in full under supporting information (Table S2).
The 4-locus sequences of the isolates between the 2 continents were identical.
As a result, the locus sequences of 263 of the 1,269 SSRs matched the cotton SSR database; therefore, 1,006 or 79.28% of the 1,269 SSRs were novel.
Primers were designed based on the locus sequences of A. nidulans FGSC A4 obtained from The Broad Institute's homepage (www.broadinstitute.org).org
To elucidate the relationship between them, we investigated the epidemiology of CC4821 serogroup B strains, characterized the outer membrane protein (OMP) genes of these strains, and analyzed the genome sequences and capsule locus sequences of specific strains.
Similarly, the 4-loci sequences of E and K isolates between the 2 continental samples were identical, indicating recent migration of these clonal groups as well.
High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and mini-satellite loci.
Individual genetic profiles are produced through a combination of 10 microsatellite loci, sequencing part of the mtDNA control region, and a mysticetes sex marker (Supporting Text S1).
The presented recombination breakpoint analysis is not suitable to distinguish between intragenic recombination or reassortment of loci as sequences of individual loci are too short to perform the tests on each locus separately.
