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Thus 2LTR circles are a much-used marker for nuclear entry.
Samples were counterstained with DAPI (4′,6-diamidino-2-phenylindole), a marker for nuclear detection.
To test out this idea, immunofluorescence staining was performed against Survivin and mAb414, marker for nuclear pore protein complexes (NPCs).
The constructs 35S::GmFLD-YFP and 35S::GmLDL2-YFP were used respectively to co-transform rice protoplasts with 35S::Ghd7-CFP, a marker for nuclear localization [ 36].
SRSF2 is a well-known marker for nuclear speckles, nuclear domains implicated in the storage and supply of splicing factors to active transcription sites (Spector and Lamond, 2011).
In order to distinguish between the two nuclei, kar1-1 cells also contained Nup82-mCherry as a marker for nuclear pores.
Similar(49)
Histone H3 and β-actin were used as markers for nuclear and cytosol proteins, respectively.
Since the nucleolus is a sub-region within the nucleus, nucleolar accumulation serves as a marker for impaired nuclear export.
To better detect the relative distribution of cytoophidia, we generated a strain expressing CTP synthase-YFP and mCherry-Ish1, a marker for the nuclear envelope.
As a further marker for necrosis, nuclear release of the yeast HMGB1 homolog (Nhp6Ap) was monitored by epifluorescence microscopy of endogenous chimeric fusion protein Nhp6Ap-GFP upon WT-Pep4p overexpression.
The phosphorothioate substitution also provides a marker for P nuclear magnetic resonance (NMR) studies, because the sulfur atom induces a 50 60 ppm downfield shift in the P signal from the substituted site.
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