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Regarding cataract subjects, mean LOCS III score for nuclear opalescence was 3.1 and LOCS III score for nuclear color was 3.3.
The multiplication of these two values gave the final score for nuclear staining: 1 2, weak; 3 4, moderate; 5 6, strong.
The score for nuclear β-catenin expression was as follows: negative - 0, < 30% - 1+, 30-60% - 2+, > 60% positive cells - 3+.
In the tumour site, all biopsies had a high (3) score for nuclear hnRNP A2/B1 and (with the exception of one) low cytoplasmic hnRNP A2/B1 staining (see examples in Figure 3d and 3f).
We then established a score for nuclear colocalization of PR and p-c-Jun in which 0 represents faint or no colocalization in less than 10% of cells, 1+ weak colocalization in 10 to 25%, 2+ moderate colocalization in 26 to 50%, and 3+ strong colocalization in >50% of cells.
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The cells were then fixed and scored for nuclear translocation of PIR.
Blinded samples were reviewed by a pathologist and scored for nuclear grade and Van Nuys classification.
The sections were scored for nuclear ATRX positivity by two independent observers blinded to the clinical data.
P63 was scored for nuclear staining only, and no significant labeling of any other cell compartments was seen.
The sections were scored for nuclear positivity based upon distribution (0, 0 25, >50) and intensity (light, strong).
Images of ≥100 cells per chamber manually scored for nuclear localization using the blue dapi counter stain as a nuclear localiser.
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