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About 300 mm circular area was then marked and the full thickness of the marked area was carefully excised by using sharp sterilized scissors.
Repeated whole-body PET scan of the marked area was initiated at the time of the tracer injection.
Following this, the length of TH-positive axons within the marked area was measured.
Using a 0.6 mm-diameter stylet, one core from each marked area was transferred to a recipient block.
The marked area was completely scraped off the slide using a pipette tip, and neoplastic tissue or normal tissue was collected into different microcentrifuge tubes.
If the laser did not succeed in releasing the tissue completely, the resultant marked area was carefully removed by an experienced dissection microscopist and placed directly into RLT buffer.
Similar(54)
Close-ups of the marked area are shown in (b) and (c).
Snapshots of each marked area were taken over a period of time.
In rare tumour cells, the exact locations of the tumour cells in this marked area were saved with relocalisation software (Mark&Find Module; Carl Zeiss Vision GmbH, Halbermoos, Germany) linked to an automated stage (type 00-24-473-0000; Carl Zeiss AG, onerkochen Germany) on a Zeiss Axioplan 2 epifluorescence microscope (Zeiss, Jena, Germany) before hybridisation.
Through microscopic examination of histologic slides, representative tumour portions were marked on the tissue slides, and then the marked areas were subjected to manual microdissection.
The line profiles that were extracted from the phase images and the HAADF-STEM image along the marked areas are given in Fig. 7 Fig. 7 Phase variations on solar cell with CIGS of high Cu content (a, b) Intensity line profiles showing the phase variation (green lines) which is extracted from the regions A (_{1}), A (_{2}), B (_{1}), and B (_{2}) marked in Fig. 6a, b.
Related(20)
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