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The marked slide was used to guide microdissections.
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The marked slides were scanned onto the Ariol SL50 system (Genetix, Gateshead, UK) for ease of comparison with the cut surfaces of the paraffin blocks and 0.6 mm cores of the marked areas were taken using a Beecher Manual Tissue Arrayer 1 (Beecher Instruments, Sun Prairie, WI, USA).
Pre-marked slides were then micro-dissected using a Leica TM LMD6000 system.
The slide was mainly caused by a marked drop in soyoil prices, reflecting expectations of ample global soybean supplies, notwithstanding lower than earlier anticipated production in the United States and Brazil.
Slide was scanned immediately.
A slide is $249.
A smoother slide is a safer slide.
The marked slide and paraffin-embedded tissue block were aligned so that the targeted area of tumour on the donor block could be determined.
Areas corresponding to the marked regions on the H&E slides were mapped onto the immunostained slides.
Slides were pre-marked for benign or malignant areas and by Gleason grade (Grades 3/4/5) using the diagnostic matching slide as a guide.
Tissue punches 1 mm in diameter were obtained from an area of the FFPE tissue block corresponding to the marked slide.
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