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The '232' × '1392' F1 mapping population, comprising 95 progeny lines, was used in this study.
A Doubled Haploid (DH) mapping population comprising 129 individuals was developed from the cross between two UK hexaploid winter wheat cultivars (Triticum aestivum L ., Spark and Rialto.
The mapping population comprising 180 backcross progeny clones (hereafter referred to as DMDD) was raised in the greenhouse for DNA extraction and pathogen testing and is also maintained pathogen-free in vitro (https://research.cip.cgiar.org/confluence/display/dm/Home) at the International Potato Center, Peru.
Our model derivation is based on a mapping population comprising of n recombinant inbred lines (RILs), initiated with two inbred lines.
These include a genetic linkage map and mapping population comprising ~500 doubled haploid lines (DHLs) [ 53], which will allow efficient de novo sequencing and high-quality assembly, leveraging, for example, the recombinant population genome construction approach of Hahn et al. [ 95].
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For example, an SNP-based linkage map constructed using M432 Malus mapping population comprised 2272 SNP markers, 306 SSR markers, and the S-loci, the average distance between markers was increased to 0.5 cM [ 5].
Unfortunately, as is the case with any mapping population comprised of unique individuals, direct replication of the experiment is not possible.
Finally, once the contaminants were removed, the mapping population comprised 161 individuals: 106 from family C14xC15 and 55 individuals from C15xC14.
The mapping population comprised 161 F5 RILs derived by selfing of single F2 plants described previously (Lin et al. 1995) from a controlled cross between single plants of S. bicolor BTx623, and S. propinquum (unnamed accession).
Genotyping was carried out on three mapping populations comprising 177, 80 and 90 F1 plants from the P1, P2 and P3 pedigrees, respectively.
The only significant omissions from the Collection were three mapping populations comprising recombinant inbred lines prepared from JI accessions JI399 and JI15 or JI281 and JI 15 and JI 1194.
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