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Principal component analysis (PCA) was performed to visualize the various branching types (e.g., apical vs. basal branching) within the SAM association mapping population using the pca function implemented in the FactoMineR package ver. 1.16 [ 34] available in the R statistical computing language (ver. 3.1.0) [ 35].
A genetic map was produced for each mapping population using JoinMap software to estimate linkage, map order, and distance.
SNP typing of the entire mapping population using NGS technology has also been applied in polyploid wheat species.
Prediction abilities of models were evaluated both for the whole NAM panel and for within each of the 25 biparental mapping population using a cross-validation procedure.
Total RNA was extracted from young leaves from GF and UN (the two parents of the mapping population) using a CTAB RNA extraction method [ 20].
This new microarray containing selected probes was used to genotype an additional set of 68 individuals drawn from the mapping population using a Selective Mapping approach.
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The mapping population used in this study was derived from a cross between two distinct botanical varieties [6].
Being nonpolymorphic, they could not be genetically mapped on the tetraploid mapping population used here.
Note that the D17SS F12-AIL is also the mapping population used in the present study.
A mapping population used to map QTLs in hybrid poplars (Populus trichocarpa Torr. and A. Gray (T) × Populus deltoides Bartr.
RBT developed the Li 2 mutant and WT NILs and the F2 mapping population used as plant material.
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