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Infection of a double haploid wheat mapping population segregating for Snn1 with P. nodorum SN15 identified Snn1 as a major SNB QTL at both growth stages, based on the severity of tissue necrosis.
A total of 16 unidirectional cDNA libraries were constructed from all the four genotypes i.e. 'ICPL 20102' and 'ICP 2376'ICPICP 7035' and 'TTB 7' which represent parents of mapping population segregating for FW and SMD, respectively.
Highly susceptible (9621-94) and resistant (9621-67) genotypesotypes were selected from a mapping population segregating for resistance to X. fastidiosa.
Two chickpea genotypes, ICC 4958 (drought tolerant) and ICC 1882 (drought sensitive), parents of a mapping population segregating for drought tolerance were selected.
In Arabidopsis, a PAP gene was also shown to underlie a QTL for oil content in a mapping population segregating for this trait [ 33].
For instance in chickpea, Solexa 1 Gbp technology was used to sequence root cDNAs from parents of a mapping population segregating for drought tolerance [ 32].
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Additionally, as our mapping population segregated for melting/non-melting flesh (MnM) this trait was also included to analyze if there is a possible pleiotropic effect of the locus that controls flesh type on volatile production.
In addition, 38 inbred parents of mapping populations segregating for drought, downy mildew and rust (Supplementary Table 41) and 31 wild accessions representing seven countries (Mali, Mauritania, Senegal, Sudan, Chad, Mali and Niger) were also resequenced using the WGRS approach (Supplementary Table 42).
Further experiments involving mapping populations segregating for Xa4 and xa5 would be needed to test this hypothesis.
We first screened the parents of two mapping populations segregating in tolerance to Fe pulse stress, subsequently conducted two QTL mapping experiments with these populations, and finally investigated the physiological mechanisms underlying tolerance using selected lines from the mapping populations.
The identification of two putatively novel QTLs and their possible interactions with other Xa genes needs further validation in mapping populations segregating for Xa4, xa5, QTL-1 and QTL-12.
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