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Our general model allows us to screen over many different putative SAC networks, corresponding to different assumptions about which molecular species participate in the inhibitory complexes, which proteins facilitate the formation of these complexes on the kinetochore, and the means by which these complexes inhibit Cdc20.
Within this sequence many different putative binding sites for nuclear factors were predicted in silico.
The small number of RPG upstream regions containing many different putative TF-binding motifs may suggest that all four sites are not necessary for regulation of the RPGs.
As each miRNA can have many different putative target mRNAs, a miRNA can essentially be the central tumorigenic factor [ 8] or it can function through inhibiting mRNAs that in turn function as either oncogenes or tumor suppressors [ 9, 10].
Among the many different putative targets for these miRNAs, we discovered two mRNAs encoding for proteins with a relevant function in myogenesis: the Myocyte-specific enhancer factor 2C (MEF2C), targeted by miR-135 and Mastermind-like-1 (MAML1) controlled by miR-133.
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Using exclusively these overstrict criteria, we have been able to identify as many as 64 different putative SFPs (Table 1).
In this work we have explored the possibility of constructing a new chemically synthetic operon to transfer the capacity to produce isobutanol to different bacteria, allowing the possibility to test in a simple way many different host organisms as putative isobutanol producers and to regulate it in an integrated manner.
However, in TNFα signaling, the putative redundant or nonredundant involvement of many different Ub chain types leads to a complicated interplay of Ub signaling (Kulathu and Komander, 2012).
This paradigm is being confirmed by the work of many different laboratories as our understanding of posttranscriptional regulation broadens and putative operons are described [ 8, 13- 17].
Later on, Rodionov and Gefland reported the presence of putative NrdR boxes upstream from nrd genes in many different species of microorganisms [22].
As shown in Table S2, the putative target RNAs encode proteins that function in many different processes including neurotransmitter biosynthesis, synaptic transmission, membrane excitability, nuclear/cytoplasmic transport, signal transduction, cell adhesion, cell proliferation and cell death.
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Justyna Jupowicz-Kozak
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