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Peptides of interest were identified manually by searching their m/z-values within the experimental mass spectrum.

Mass spectral peaks of biological origin were identified manually by searching for mass shifts between C- and C-mass spectra.

Functional analysis of tRNAs/tDRs was carried out manually by searching online due to the lack of a functional database for tRNA.

Determination of a complex's novelty is done manually by searching for the presence of lipid-associated annotations or references in the literature.

Mass spectral peaks of biological origin were identified manually by searching for mass shifts between the C and C mass spectra.

QTL hotspots were identified manually by searching in a sliding window of 20 cM in the original QTL data and the regions with more than three co-locating QTLs in each window region were recorded.

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Similar(52)

A series of fragments, representing the compounds with greater uncertainty, were manually identified by searching among the structures with highest error (greater than 1 log unit) or misclassified (predicted nB when they are vB, or vice versa).

Of the 60 genes that passed the 15% FDR and the two-fold cutoff, 55 either had pre-assigned Human Gene Nomencltaure Committee HCGNN) approved gene symbols in the data output, or the gene symbol could be manually assigned by searching BLAT in the UCSC genome browser using the probe sequence.

The functions of the genes were further manually updated by searching the GenBank with BLASTX.

Annotations were manually validated by searching for protein domains in Pfam by hidden markov models (http://pfam.sanger.ac.uk/).sanger.ac.uk/

All of the peptides were manually validated by searching a non-deuterated protein sample's MS scan to test for correct m/ z state and to check for the presence of overlapping peptides.

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