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Topic model results were analysed manually by inspecting the top relevant words (i.e., 20 per topic).
Identified sequences were confirmed manually by inspecting the fragmentation spectra.
The onset latency of calcium events was computed manually by inspecting each airpuff-evoked calcium event.
This process is done manually by inspecting additional information like sequence similarity or functional information about the involved entries.
We therefore improved the locus order manually by inspecting graphical genotypes (see Methods section entitled "DArT skeleton bin map").
Trials contaminated by eye-blinks or movements were removed manually by inspecting the amplitude time courses of the raw eye-channel data.
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Gene definition was manually annotated by inspecting BLASTP results and sequence alignments.
To circumvent problems such as false positives, larger inserts, or missed large deletions, all detected variants were manually curated by inspecting the regions and the aligned reads.
The start and end values of the six features for an ideal intercalation were manually defined by inspecting several clear intercalation events in the data set.
We controlled for this by inspecting manually every duplication case to discard dubious cases.
We only considered peptides with double and multiple charges, and manually evaluated the expression values by inspecting the areas of integration that the software had chosen and by adjusting them as needed.
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