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Spots were enumerated manually by inspection under a dissecting microscope, and automatically using an ImmunoSpot Analyzer-BioReader 4000 Pro-X (BIO-SYS GmbH, Karben, Germany).
We note that the gene repressions for this group of GSLs can be inferred either manually by inspection of the GPR associations for each inconsistency (as we did for GNGs), or automatically, by using the modified version of the GrowMatch (see Methods).
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Epochs with eye movement or other movement artifacts were manually removed by inspection.
Assignments were manually verified by inspection of the tandem mass spectra and coalesced into Scaffold reports (www.proteomesoftware.com).com
Results were initially filtered using only XCorr and mass accuracy, and candidate phosphopeptides were manually verified by inspection of the corresponding tandem mass spectra.
Protein and peptide identifications were further analyzed and manually verified by inspection of chromatograms and spectra.
The quality of the resulting contigs was manually confirmed by inspection using the CLVIEW cluster viewer program.
All but one identified peptide had a minimum Mascot ion score of 20, and all sites of predicted PTM were manually validated by inspection of the MS/MS spectra.
Meanwhile, partially-shelled and unshelled seeds could only be separated manually by visual inspection.
Heterodera avenae and other cyst-producing nematode species are traditionally counted manually by visual inspection, a laborious and time consuming process.
P-wave first-motion polarities were selected manually by careful inspection.
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