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Usually the process parameters are manually adjusted on the introduction of new production scenario or after the deviation between the actual form of produced pieces and the designed form become clear.
Structures were iteratively refined with Refmac after working models were displayed in Coot and manually adjusted on the basis of electron density maps.
After the alignment is manually adjusted on the basis of this observation, models B and C show better fits for fragments 45 49, 143 149, and 169 172, as these regions are predicted to be helices in helix bundles.
Intron prediction was performed using SpliceView (http://www.itb.cnr.it/webgene/) with an option of organism=" Caenorhabditis elegans" or organism=" Aspergillus niger" for nematodes and fungi respectively and manually adjusted on the basis of conserved regions of GH45 cellulase sequences.
GenomeScan [ 43] was used to predict coding exons from sequences derived solely from WGS or genomic scaffolds, some of which were manually adjusted on the basis of known intron-exon boundaries in other organisms.
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The position of the metal tube can be manually adjusted for focusing the laser on the back of the cantilever.
Subsequently, PRC and PHC masks were coregistered and resliced to the individual mean EPI images and manually adjusted to achieve a precise overlay on the functional data.
Each image was manually inspected by one researcher (MY) and manually adjusted to minimize segmentation errors.
Alignments were then manually adjusted to improve the overall alignment.
The sequence alignment was manually adjusted based on known secondary structures.
Coding sequences of these Drosophila species were predicted from genomic regions containing BLAST matches using the programs Genscan [18] and SNAP [19], and manually adjusted based on ortholog alignments.
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