The number of motor neurons on each section was manually quantified in ImageJ.
To gain further insights into these differences we manually quantified the evolution of AS cell shape changes using three parameters: apical surface area, elongation and membrane content (Figure 5A and for details on analysis see Materials and Methods).
Crystal violet solution was then washed off and colonies were manually quantified.
The positive cells were then manually quantified in 10, 40 × power fields from each sample.
To quantify proliferating endothelial cells, tissue sections that were dually stained for VWF and Ki67 nuclear proliferation antigen were manually quantified.
Acquire multiple exposure times to manually quantify every visible metastatic focus.
Of course, manually quantifying what constitutes a 'large' deviation is very hard to do.
Calbindin-positive INL cells were quantified on retinal cryostat sections manually from optic disc to periphery.
TUNEL-, ICAM-1 and Ki67-positive cells were quantified manually, using a microscopic grid on images under ×400 magnification.
A total of 40 HIV-1 pol PCR assays were performed on FP-DBS spiked in duplicate (A and B) with known copies of HIV-1 in ACH-2 cells (manually counted and quantified by HIV-1 pol real-time PCR assay done) on two different days (D1 and D2).
