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Exact(11)
yeast malt broth.
Yeast malt broth media constituents (peptone, yeast extract, malt extract; dextrose) and phenyl methane sulfonyl fluoride (PMSF) were purchased from Hi Media laboratories.
Cadmium has been found to be accumulated to a higher concentration of 20 mg/g dry weight in P. ostreatus when grown in liquid cultures of malt broth (Favero et al. 1991).
The growth of this yeast in yeast malt broth media (YMB) shows the lag phase (0 6 h), exponential/log phase (6 30 h), stationary phase (30 42 h) and decline phase (42 h and above) (Fig. 1).
Mycelia of G. striatum DSM 9592 were grown in a fourfold diluted malt broth, washed and then re-suspended in a defined mineral medium to give a concentration of about 2 mg dry weight per mL, as has been described previously (Wetzstein et al. 1997).
Fig. 1 Growth curve of Candida parapsilosis ATCC 7330 cultured in a shake flask using Yeast Malt Broth media Fig. 2 a UV/vis spectrum analysis of the gold nanoparticles biosynthesized using whole cells (24, 36 and 48 h culture cells) of Candida parapsilosis ATCC 7330, b visual representation of the reaction.
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Fungal cultures were grown in malt extract broth (2 g malt extract in 100 ml water).
The assays were carried out in a laboratory medium (Malt Extract Broth).
Malt extract broth (MEB) is the commonly used medium in our laboratory for fungal cultivation.
The mycelia were then transferred to glucose yeast malt peptone broth (GYMP).
The spore suspensions were added to the 250-mL Erlenmeyer flasks containing 50 ml of nutrient media (Malt Extract Broth).
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