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The sediment was reconstituted to 2.5 ml with phosphate buffer pH 6.8, to make the inoculum for the smears and cultures.
The sediment was reconstituted to 2.5 ml using a phosphate buffer (pH 6.8), to make the inoculum for the smears and cultures.
The sediment was then reconstituted to 2.5 mls, using phosphate buffer pH 6.8, to make the inoculum for smears and cultures.
One ml of culture was taken from each flask (where growth was not observed) for serial dilution to make the inoculum of 106 cfu/ml and inoculated on PDA plates and incubated at 30°C for 48 h.
The sediment was reconstituted to 2.5 ml with phosphate buffer pH 6.8, to make the inoculum for the fluorescent smear microscopy, and cultures in the automated MGIT (Becton Dickson) liquid culture system were incubated at 37°C for at least six weeks.
Similar(55)
Sediments were reconstituted to 2.5 ml with phosphate buffer pH 6.8, to make the inoculums for the cultures.
The sediment, irrespective of the original sample volume, was reconstituted to 2.5 ml with phosphate buffer pH 6.8, to make the inoculums for the smears and cultures.
Nonfat, dried, low-heat-grade milk without added vitamins A and D (Dairytown Products Ltd ,Sussex, NB, Canada) was rehydrated (12% wt/vol) and autoclaved (115°C for 15 min) to make the inoculums.
The finding of largely the same Fusarium species on barley, oats and wheat (Bernhoft et al. 2010) may indicate that any of these cereal species may make the Fusarium inoculum correspondingly available for the crop produced next year.
The intended number of cfu was extrapolated from a standard growth curve, and appropriate dilutions with sterile PBS were made to prepare the inoculum for the mice.
The inoculum was spread out slightly with the intention to make the coverage on all the coupons and in the "control" Petri dishes alike.
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