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Similarly, we found that filopodia containing clusters of Shank1b were less dynamic and led to an increase in the number of spines formed [5], suggesting that these filopodia function to make stable contacts, consequently leading to the formation of a synapse.
Indeed, under certain conditions, they are able to make stable contacts with alary muscles in more anterior segments than normally occurs in wild-type.
Consistent with this view, when all the normal muscle targets are ablated, tip cells can make stable contacts with the A2/A3 alary muscle.
In other contexts where epithelial tissues make stable contacts with muscles, integrin-mediated adhesion plays a crucial role (Bökel and Brown, 2002; Levi et al., 2006; see Schejter and Baylies, 2010).
However, as anterior alary muscles (A1/A2 to A3/A4) are variably missing in Ubx 9.22 mutants (LaBeau et al., 2009), we also analyzed the phenotype of dsparc mutant embryos in which alary muscle development is unaffected but the reception of guidance cues is lost (Bunt et al., 2010) and, just as in Ubx 9.22 mutant embryos, the tip cells make stable contacts with the A5/A6 alary muscles.
Similar(55)
Pipettes using P2, P3 and P4 were backfilled with 15 mM Cl- substituted for methanesulfonate in order to make stable contact with the Ag/AgCl electrode.
In yeast and mammals, most evidence suggests that the proteasome lid assembles completely before making stable contact with the base complex or its subunits (Fukunaga et al., 2010; Thompson et al., 2009; Tomko and Hochstrasser, 2011).
They help make stable, diverse societies possible.
AFF4 meanders over the surface of the P-TEFb cyclin T1 (CycT1) subunit but makes no stable contacts with the CDK9 kinase subunit.
Work on making stable approaches.
In addition, live imaging of green fluorescent protein (GFP -labeled dendrites in acute hippocampal slices from P10–12 mice showed that spines sample nearby synaptic resources even after makinGFP -labeledontact (Konur andendrites2004).
Related(20)
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