Sentence examples for make stable transfectants from inspiring English sources

Both types of lentiviral particles were used to transduce lymphoid (Daudi) and epithelial (Hela) cells in order to make stable transfectants.

To test the contribution of cyclin D1 downregulation to the G1 arrest, we made stable transfectants of MCF-7 and MDA-MB231 cells with forced overexpression of cyclin D1 (the expression plasmid was kindly provided by Joshua D Liao, The Hormel Institute, Austin, MN, USA).

Transfected cells were passaged six times in a selection medium to produce stable transfectants.

Van Helvoort et al. have made the stable transfectants of pig kidney epithelial LLC-PK1 cells expressing human ABCB4, which is localized in the apical membranes [ 34].

Work on making stable approaches.

At 24 h after transfection, stable transfectants were selected with 10 μg/mL puromycin (Life Technologies).

We adopted a pooled stable transfectant strategy as we have used previously to minimize the effect of integration site bias; however, because of potential selection bias in pooled stable transfectants, this entire stable transfection procedure was independently repeated twice more.

Stable transfectants of KG-1 made with cDNA for HLA-B*2705 HLA-B*2705ithout the C-terminal sv5 epitope tag [ 16] with generandd using the Amaxa Nucleofector (Amaxa AG., Cologne, Germany).

To obtain stable transfectants, the pC28-GUSh was transfected into CHO cells by Lipofectamine Plus reagent (Life Technologies).

Pooled stable transfectants carrying either construct were used to minimize any potential effect of clonal selection.

Hollow fiber production of stable transfectants was established to produce the fusion protein.