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However, 3DP has major limitations, such as limited resolution and accuracy, hence preventing the widespread application of this metho engineering.

Photocatalytic reactors for the degradation of gaseous organic pollutants often suffer from major limitations such as small reaction area, sub-optimal irradiation conditions and thus limited reaction rate.

Although acid hydrolysis is a relatively fast process, it suffers from some major limitations such as high operational cost, by-product formation, corrosion of equipment, neutralization requirement, high disposal cost (Bansal et al. 2009; Wang et al. 2012).

Efforts have been made to improve the water stability of electrospun protein scaffolds using cross-linking and other approaches, but the current methods have major limitations, such as cytotoxicity and low efficiency.

This system has major limitations such as rapid drug release caused by physical instability and high solubility of Ca-alginate beads in neutral and weak alkali media (George and Abraham 2006; Ma et al. 2010; Tavakol et al. 2009; Xing et al. 2003).

However, in the diagnosis of early CRC it has major limitations such as low sensitivity and specificity (36%and87%7% respectively).

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A major limitation such a photoreactor is a part of the reactor volume expose to UV radiation.

We also underline the major limitations of such genetic studies, as a complement to what was previously explained by Blench et al. (2008).

One of the major limitations of such approaches is that they are unable to identify "local" similarity between structures thereby increasing the false-negative rates (i.e. identifying cases with weaker similarities).

While this approach does not overcome the major limitations of such models, introducing a rank order of selected features may allow selecting model cell lines that are more adapted and pertinent to the addressed biological question.

However, one of the major limitations for such kind of approach is that the forms of vaccines compared may not be optimized, including the conditions used for HPV pseudovirions.

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