The present work established an efficient staining method for fluorescence activated cell sorting (FACS) with Chlorococcum littorale maintaining cellular viability.
Thus, the newly developed VS442 vitreous solution was demonstrated to be more efficient in maintaining cellular viability and osteogenic function for vitreous cryopreservation of TEB over VS55.
One explanation is that the ATP level in the FCCP treated cells was already nearing a lower limit necessary for maintaining cellular viability.
For these functions, the microtubules and many associating proteins in these complexes, require correct spatial and temporal organization to allow proper localization and dynamics of the MTOC and the microtubules for maintaining cellular viability and integrity[29], [30].
Similarly, HDAC III activity can have both pro- and antiapoptotic effects depending on cell type and proliferation state, and the right balance between the activities of different HDAC classes therefore seems to be crucial for maintaining cellular viability.
In normal cell lines, the number of DSBs evoked by a certain amount of stress is largely constant and the outcome is mediated by the efficiency of the DSB repair, whereas, in cancer cells, the increased total number of DSBs may present a major challenge for maintaining cellular viability.
