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Surprisingly, positive supercharging maintained wild type activity levels in ILs, while negative supercharging drastically reduced activity.
In contrast to random PEGylation of the enzyme by NHS-ester mediated chemistry, controlled mono-PEGylation of A1-III alginate lyase produced a conjugate that maintained wild type levels of activity towards a model substrate.
To ask whether this Ca2+ conducting channel was responsible for calpain activation and the migration of T cells, we used a human T cell line derived from a immunodeficient patient homozygous for a non-functional mutated ORAI1-R91W protein and compared its migratory potential with a similarly maintained wild type control T cell line [34].
In the text we say: "This effect was specific to response timing, because AC males maintained wild type levels of song production, and showed no change in inter-bout interval (IBI) or pulse frequency.
Similar(56)
Amongst a collection of 28 strains carrying deletions in genes for glycosylphosphatidyl inositol (GPI -anchored proteins, the Δsed1 and Δtos6 strains had siGPI -anchoredigher BGL-activity whilst maintaining wild typroteinsh.
The vector we employed encodes a fusion protein (GFPBcl-xL) previously shown to maintain wild type Bcl-xL biological function [38].
Specifically, the impact of accumulating abasic sites was examined by (i) increased expression of Mag while maintaining wild type levels of Apn1 (WT/pMAG), (ii) maintaining wild type levels of Mag in an Apn1 deficient strain (Δapn1), or (iii) increased expression of Mag in an Apn1 deficient strain (Δapn1/pMAG).
Our strategy renders mice heterozygous at the RIα locus, and expression from a single wild-type RIα allele appears to be adequate to maintain wild type PKA activity levels in most tissues.
No measurable changes were observed in kinase activity from ES cells harboring the RIαB allele compared to WT ES cells, suggesting that a single allele is sufficient to maintain wild type levels of PKA activity in ES cells (Figure 2C, RIαB lane).
YPD medium (1% yeast extract, 2% peptone and 2% D-glucose) was used to routinely maintain wild type strain.
Despite the dramatic apoplastic bypass in sgn3 roots, the mutants managed to maintain wild type levels of sodium (Na), calcium (Ca), or boron (B), for example.
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