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Furthermore, they induce a partial rejection of unmodified, less immunogenic established wild type tumor cells [ 82, 83].
We utilized established wild type and DAXX null fibroblast cell lines and a combination of imaging-based techniques including immunofluorescence, fluorescence in situ hybridization (FISH), and correlative electron spectroscopic imaging (ESI), a specialized form of energy-loss transmission electron microscopy.
Features of Tau4RTg2652 mice distinguishing them from other established wild type tau overexpressing mice include very early phenotypic manifestations, non-progressive tau pathology, abundant pre-tangle and phosphorylated tau, sparse oligomeric tau species, undetectable fibrillar tau pathology, stability of tau transgene copy number/expression, and normal lifespan.
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The reporter cell line was generated by infecting U-2 OS cells with lentivirus encoding an established wild-type p53-Venus reporter construct [ 9].
Injection of glioblastoma cells (C6) IGF-1R antisense cells into rats carrying an established wild-type C6 tumour caused complete regression of the tumours.
Between 28 and 32 hpf (hours post fertilization), the paired ventro-lateral primordial hindbrain channels (PHBC) and primordial midbrain channels (PMBC) that extend along the rostro caudal axis, establish wild-type-like connections with the medial basilar artery (BA) and the more rostral V-shaped posterior communicating segments (PCS).
Antigen-induced arthritis (AIA) was established in wild type (WT), gp130F/F mice displaying hyperactive gp130-mediated STAT signalling and the compound mutants gp130 F/F:Stat1 −/− and gp130 F/F:Il17a −/− mice.
PCR genotyping (Fig. 1D) established that wild type, heterozygotes (Nrp1 +/cytoΔ) and homozygotes (Nrp1 cytoΔ /Δ) were present at the expected Mendelian ratio (see Table S1 in the supplementary material).
Primary adult hypothalamic astrocyte cultures were established from wild type rats and mice, estrogen receptor-α knockout (ERKO) mice, and four core genotype (FCG) mice, with the sex determining region of the Y chromosome (Sry) deleted and inserted into an autosome.
Claren, J., Malisi, C., Höcker, B. & Sterner, R. Establishing wild-type levels of catalytic activity on natural and artificial 8-barrel protein scaffolds.
We have previously established that these wild type islets do not constitute a target for autoreactive T cells, since they lack both the costimulatory CD80 molecule and the GP autoantigen.
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