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Tg2576:sod2 mice, Tg2576 mice, and C57BL/6:SJL F1 hybrid sod2 heterozygotes were maintained under barrier conditions until 497 527 days of age, when they were killed and neocortices were either harvested and frozen for biochemical analysis, or fixed in neutral buffered formalin for histological processing using standard protocols.
All mice were maintained under barrier conditions and were pathogen free as assessed by regular microbiologic screening.
Inbred 8- to 12-wk-old C3H/HeN, C3H/HeJ (Harlan), BALB/c (The Jackson Laboratory) and C5L2KO (on BALB/c background) mice were maintained under barrier conditions and pathogen free.
Male BN rats (SubstrainBN/SsNOlaHSD) were obtained from Harlan UK at 21 28 days of age and maintained under barrier conditions on a 12 h light: 12 h dark cycle (08 00 20 00).
All the animals used in this study (5- to 6-week-old female athymic Ncr nude mice) were purchased from Taconic (Taconic, Hudson, NY, USA) and maintained under barrier conditions (pathogen-free conditions provided by plastic cages with sealed air filters).
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Guinea pigs were maintained under ABSL-3 barrier conditions in isolator cages (Thoren, Hazleton PA).
(Kyoto, Japan) and maintained under pathogen-free barrier conditions.
All mice were maintained under standardized non-barrier conditions in the Laboratory Animal Facility of the Istituto dei Tumori di Napoli (Naples, Italy) and all studies were conducted in accordance with Italian regulations for experimentations on animals.
The mice were maintained under specific pathogen-free barrier facility at the Institute of Biophysics, Chinese Academy of Sciences.
C57BL/6n mice (6 8 weeks old) were purchased from Charles River Wiga (Sulzfeld, Germany) and maintained under specific pathogen-free barrier conditions at the Central Animal Laboratory (Nijmegen, The Netherlands).
Mice were housed in a barrier facility maintained under standards meeting federal, state, and local guidelines and under the supervision of licensed veterinarians.
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