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The animals were maintained under isoflurane inhalation anesthesia during radiotracer administration and imaging.
As previously described (Pollot and Corona, 2016; Greising et al. 2017), swine were anesthetized with Telazol (4 6 mg/kg, i.m ., intubated and maintained under isoflurane anesthesia.
Mice were maintained under isoflurane anaesthesia during radiotracer administration, scanning of either radiotracer (induction 5%, maintenance 2%, with 70%N2O/30%% O2, Minerve anaesthesia system, Bioscan, France).
SCID mice (6-to-8-week-old male, Charles River Charles Riverr, NJ, USA) were maintained under isoflurane anaesthesia during surgery.
The animals were maintained under isoflurane (1.5-2.5 1.5-2.5hesia throughout imaging.
Capsaicin (50 µl) was administered as 0.3% solution (dissolved in 10% ethanol, 10% Tween 80 and 80% saline) instilled into the colon via a Hamilton syringe catheter (rounded-tip needle 3.8 cm long) while the mouse was maintained under Isoflurane anesthesia.
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While the mice were maintained under 2% isoflurane anesthesia, Matrigel was directly injected into the subcutaneous tissue of the left ear, and a 400 pM GNR Matrigel solution was injected into the right ear.
Mice were anesthetized with 2 3% isoflurane and maintained under 1.0 1.5% isoflurane in 70% N2O and 30% O2 using a facemask.
For live imaging of bioluminescent bacteria in tumor-bearing mice, animals were anesthetized with 4% Isoflurane (Forene, Abbott) and maintained under 1% Isoflurane in a thermostated chamber with a high-resolution charge-coupled-device (CCD) cooled digital camera ORCA-2BT (Hamamatsu Photonics).
Mice were maintained under anesthesia using isoflurane during pulmonary function testing.
The rats were induced and maintained under anesthesia using isoflurane.
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