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This culture was maintained under lab condition for several generations before starting the experiments.
Cx. p. quinquefasciatus used in this study were from a laboratory colony originating from adult mosquitoes collected in Merced, CA in the 1950s and maintained under lab conditions at the Kearney Agricultural Center, University of California, as previously described [23].
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Clones were maintained under standard lab conditions for at least 3 asexual generations prior to the start of the experiment to minimize maternal effects [ 47].
Plates were maintained under controlled laboratory conditions.
Although lab-reared flies are generally maintained under relatively standard conditions of temperature, light, and diet, selective pressures in the lab are very different than those found in the wild providing the potential for strong selection for human-accommodating phenotypes and/or the relaxation of selection on traits adapted in nature [ 21, 22].
BABL/c nude mice about 4 to 6 weeks old were purchased from Vital River Lab Animal Technology Co., Ltd., and maintained under specific-pathogen-free conditions in Experimental Animal Department of the Third Military Medical University (Chongqing, China).
Six-week-old female athymic nude mice were purchased from Jung Ang lab animal (Seoul, Republic of Korea) and maintained under conventional conditions.
Male athymic nude mice (NCr-nu) were purchased from Sino-British SIPPR/BK lab Animal Ltd., Co (Shanghai, China, license No. SCXK 2010 0002) and maintained under specific pathogen-free conditions for the studies.
Ex vivo experiments were conducted on cells grown on Lab-Tek chambered coverglass (Nalge Nunc International) and maintained under standard culture conditions (37°C, 5% CO2).
Mice were maintained under specific pathogen-free condition at the facility of Janssen Research & Development and the Jackson Lab.
The goat was maintained under general anesthesia.
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