Sentence examples for maintained in proliferation from inspiring English sources

The cultures were maintained in proliferation medium consisting of Neurobasal (Invitrogen), B27 or N2 supplement (Invitrogen), 2 mM Glutamax (Invitrogen), Pen-Strep (Sigma), 20 ng/ml human Fibroblast Growth Factor-2 (FGF2), and 20 ng/ml human Epidermal Growth Factor (EGF) (both from R&D or Pepro Tech).

hMADS cells were maintained in proliferation medium supplemented with 2 ng/ml fibroblast growth factor 2 (FGF2) [ 21].

In order to test this hypothesis, we decided to use N20.1 immortalized oligodendroglial cell line grown in culture conditions in which cells are maintained in proliferation as undifferentiated progenitors.

Briefly, the conditionally immortalized cells (expressing a v-myc transgene under the control of a tet-off system), maintained in proliferation medium, consisting of advanced DMEM/F12 containing 2 mM L-glutamine, 1 × N2 supplement (Invitrogen, Darmstadt, Germany) and 40 ng/ml FGF-2 in a 5%CO2/95%% air atmosphere at 37 °C, were passaged every other day.

Purified Schwann cells (95%, S100 positive) were maintained in proliferation medium containing 90% Dulbecco's Modified Eagle Medium (Invitrogen, Cergy-Pontoise, France), 10% foetal calf serum (Invitrogen, Cergy-Pontoise, France), 1 µg/ml forskolin (Sigma, St Louis, MO, USA), 10 ng/ml human heregulin β1 (RetD system) and 10 µg/ml insulin (Sigma).

The proliferation and rooting ability of the shoots derived from the capsules were compared with those maintained in normal proliferation by 45 days period of subculturing.

KG1a, K562 and HL-60 AML cell lines were maintained in exponential proliferation in RPMI 1640 (Gibco-Invitrogen, Paisley, UK) medium with 10% fetal bovine serum (FBS, Gibco-Invitrogen) and 100 units/ml Penicillin and 100 µg/ml of Streptomycin (Gibco-Invitrogen).

Thereafter the cells were washed and maintained in exponential proliferation with 20 ng/mL IL-2 (Chiron) for another 2 days.

In initial experiments, CreER T2LKB1+/+ and CreER T2LKB1fl/fl peripheral T cells were polyclonally activated with CD3 Ab and maintained in exponential proliferation in vitro by the addition of IL-2.

KG1a and K562 AML cell lines were maintained in exponential proliferation in RPMI 1640 medium (Gibco-Invitrogen) with 10% fetal bovine serum (FBS, Gibco-Invitrogen) and 100 units mL−1 of penicillin and 100 μg mL−1 of streptomycin (Gibco-Invitrogen).

Cells were maintained in oligodendrocyte proliferation media (OPM) consisting of DMEM/F12, B27 neuronal supplement, and 10 ng/ml platelet-derived growth factor (PDGF /fibroblast growth factor-2 (bFGF; Peprotech, Rocky Hill, NJ).