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All plantlets were then transferred to and maintained in multiplication media supplemented with 10 mg/L BAP and 0.025 mg/L 2,4-D for ten weeks.
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29 Whether the rainy season promotes movement of the bacteria to the more superficial layers of the soil or the humidity enhances multiplication of bacteria maintained in a viable non-culturable state in the dry season has not been determined.
We show that plasmids containing pbcen5 were both efficiently and stably maintained in transfected parasites during blood-stage multiplication, without drug pressure, demonstrating that pbcen5 can confer the function of a Plasmodium centromere.
These results clearly demonstrate that the pbCEN5 plasmid can be stably maintained in parasites independent of drug treatment during multiplication, indicating that pbCEN5 segregated evenly into daughter parasites (cells) during asexual blood-stage mitosis.
In the present study, plasmids containing pbcen5 are maintained in low copy numbers (i.e., one to two copies per cell) during blood-stage multiplication, indicating that pbcen5 also prevents the formation of concatamers by suppressing a rolling-circle replication by the addition of a centromere.
As seen in Figure 2D, only the endogenous dhfr-ts gene and the plasmid were detected in all parasite populations, both at the beginning and at the end of the multiplication period, indicating that the plasmids were not integrated into the parasite genome but were maintained in episomal form in the parasite nuclei.
After dinner, her mother used flashcards to drill her in multiplication for a few minutes.
I helped Ma with the work, ran errands and every day worked sums in multiplication looking back at the multiplication table to help me when I couldn't remember.
E is the avalanche field in multiplication.
It is best if you have exceptional knowledge in multiplication.
The DP matrices are D-discrete, and assume that lookup tables for efficient min-plus multiplications of these matrices are maintained as explained in the previous section.
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