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As a negative control an equal number of wells were maintained in expansion media for 20 days.
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MEFs were maintained in MEF expansion medium and immortalized with Mitomycin C (KYOWA Mitomycin 2 mg).
Human NPCs isolated from fetal brain tissue between ten to fifteen weeks of post-conception were maintained in neural expansion medium consisting of epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) and then switched to EGF and leukemia inhibitory factor (LIF) as described in detail previously [19], [23].
The Russell Group, which represents some of the UK's most selective universities, questioned whether quality would be maintained in such an expansion.
When hES cell derived hepatic progenitor cells were maintained in hepatic progenitor expansion medium, some cells at the periphery of the colonies erupted.
ALDH+ cells were also expanded and maintained in stem cell expansion and undifferentiation media.
Human BCSCs were purchased from Celprogen (San Pedro, CA, USA) and maintained in human BCSC expansion and undifferentiation media.
H9 hESC-derived neural progenitor cells (Millipore) were maintained in ENStem-A neural expansion medium (Millipore) on plates pre-coated with poly-l-ornithine (Sigma) and laminin (Sigma), according to manufacturer's instructions.
The region's largest economic sectors are health care and education, their growth maintained, in part, by major expansions of the Buffalo Niagara Medical Campus and The University at Buffalo.
Further, the compatibility modes are also clearly identified and maintained in the shear strain expansions in order to prevent the introduction of spurious zero-energy modes.
A role in mediating endosymbiotic interactions strongly suggests that duplicate nutrient amino acid transporters and the slimfast expansion are maintained in the genome because they diverged to fill a novel role in symbiosis.
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