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Two mice per treatment were examined and the number of CD3+ cells was quantified by counting five foci at 400× magnification in three sections for each brain sector.
Migrated cells were counted using a light microscope at 160× magnification in three random fields per well of three separate wells.
Membranes were photographed and invading cells were assayed by taking the average of the number of cells at × 40 magnification in three fields.
Cells were imaged at 20× magnification in three channels: the DRAQ7 and the PEI-QDs in fluorescence mode with excitation/emission wavelengths of 633/655 nm and 350/568 nm, respectively.
SVEC4-10 cells in DMEM containing 1% FCS were added to the upper chamber for 4.5 h and then migrated cells on the bottom of the filters fixed, stained and counted at ×160 magnification in three random fields per filter.
For quantitative analysis of apoptotic cells, standardized digital images were acquired with a color digital microscopic camera system (Leitz Diavert microscope, Leica, Bensheim; AxioCam®, Carl Zeiss, Göttingen, Germany) with a resolution of 1300 × 1030 pixel at 200 fold magnification in three randomly distinguished regions of interest (ROI) of each slide and processed with AxioVision Rel.
Similar(53)
Migrated cells were counted at 1000-fold magninication in five randomly selected fields.
Cells were counted microscopically at ×1,000 magnification in four fields per membrane.
Invading cells were counted by microscopy at × 100 magnification in five visual fields including most of the membrane area.
The number of myofibroblasts was determined at 200-fold magnification in six different sections from each mouse by two blinded examiners.
After 24 h, the ability of the cells to penetrate Matrigel basement membrane matrix was quantified and captured with ×100 magnification in ten random fields.
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