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The numbers of centrosomes and centrioles from more than 100 cells were counted under the microscope in three independent experiments and shown as mean ± standard deviation.
After washing thrice with Apoptosis Wash Buffer, the cells were suspended in 300 μl buffers and analyzed with a fluorescence microscope in three independent experiments.
Cells were counted manually under a fluorescence microscope in three random visual fields (>150 cells) and counter-stained with Hoechst to calculate the percentage of dead cells versus the total number of visualized cells.
The immunopositive cells were counted under a ×20 objective of the microscope in three different areas, and the results were calculated as the average of counts, and finally presented as cells/field (×20 objective) after each treatment.
The instructors can provide guidance if and when necessary, but the onus is on the students to work together to apply their prior knowledge of microscopy and the properties of light and of simple geometry and to start to picture what a microscopist sees down the microscope in three dimensions.
Similar(55)
It took him 18 months hunched over the microscope in two four-hour shifts a day.
After staining, cells were counted under a microscope in four randomly selected fields (magnification ×100) and results were expressed in the form of a bar graph.
The number of migrated cells on the lower surface of the membrane was counted under a microscope in five fields (100×).
Colonies greater than five cells were counted using a microscope in four independent experiments.
Adherent cells were counted by microscope in five random fields at 200×.
They were counted under the microscope in five randomly selected fields at 100-fold magnification.
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