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Sixty primer pairs were selected and tested for amplification in three diploid individuals, each representing a different species of the studied group.
Summarized in this table are distinguishable restriction enzyme cutting sites due to nucleotide substitutions introduced by long PCR amplification in three HPV58 isolates.
We then tested the primers for reproducible amplification in three giant pandas under the standard PCR conditions, with annealing temperatures altered according to the primer sequence.
This alteration is present as a high copy number amplification in three of the cell lines, 7p15-p13, 7q21, 7q31, 11q21, and 12q12, and losses in 2q35-qter, 4p16, 6q26-qter, 9q34 and 19q13.2-qter.
AccuPrime High Fidelity Taq DNA Polymerase Invitrogen Life Technologiess, Carlsbad, CA, USA) was used for PCR amplification in three individuals of nmsT2100 and five of IAST-1 as described by the manufacturer.
To demonstrate the markers' broader utility in the genus, we report cross-species amplification in three additional ecologically important species (E. camaldulensis Dehnh., E. marginata Sm., E. victrix L. A. S. Johnson & K. D. Hill).
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Other events that are commonly detected in HGSOC included RB1 mutation or deletion in six cases (#56, 19, 11, 169, 27, 80), NF1 deletion in one case (#80), and CCNE1 amplification in two cases (#29, 201).
This paper investigates plasmonic amplification in two commonly used optical configurations for fluorescence readout of bioassays – epifluorescence (EPF) and total internal reflection fluorescence (TIRF).
For determination of the ideal concentration the titration of the primers drawn in this study showed T. cruzi DNA amplification in five primer pairs at concentrations 100, 200 and 300 nM and DNA fixed concentrations at 1 ng μL− 1.
We verified MYCN amplification in ten neuroblastoma cell lines by Southern hybridization.
Of the 53 plasma samples, there was poor amplification in two, and these were excluded.
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