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Exact(5)
Jurkat T lymphocytes were grown in RPMI-1640 supplemented as above.
The lymphocytes were grown in RPMI 1640 medium (GIBCO, 52140; Darmstadt, Germany) supplemented with 10% fetal calf serum (GIBCO) and penicillin/streptomycin (GIBCO).
TK6 cells and lymphocytes were grown in suspension; lymphocyte cultures were treated with the polyvalent mitogen phytohemaglutinin (Sigma /Aldrich; St Louis, MO) as described [ 36].
These lymphocytes were grown, in the presence or absence of kefir, in complete growth medium consisting of RPMI 1640 supplemented with 20% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin for 24 hours.
Monocyte-depleted ascitic lymphocytes were grown with or without VEGF, cell lysates were prepared using 10 × 10 cells per preparation and subjected to western blotting as described before (Sambrook et al, 1989; Ziogas et al, 2012).
Similar(54)
Lymphocytes (Jurkat, CTLL-2, K562) were grown and stably transfected as described in Szabò et al, 2005.
Human Jurkat E6-1 lymphocyTe T cells (ATCC # TIB-152) were grown in RPMI medium supplemented with 10% (v/v) fetal bovine serum (FBS) and maintained in humidified 5%95%% CO2 air at 37°C.
The T-lymphocyte cell lines Sup-T1 and H9 were grown in RPMI 1640 medium.
B-lymphocyte lines prepared from peripheral blood by Epstein-Barr virus (EBV) transformation were grown in supplemented RPMI-1640 medium.
Although the disease is characterized by the presence of autoreactive T lymphocytes, there is growing evidence that B cells play a central role in the pathogenesis of SLE [ 1- 3].
When, however, it is grown in vitro and then used as an antigenic stimulant to cancer lymphocytes, a "cancerlike" result (about 15%) is produced.
Related(20)
cells were grown
lymphocytes were reported
lymphocytes were generated
lymphocytes were cultivated
lymphocytes were decreased
lymphocytes were propagated
lymphocytes were increased
lymphocytes were expanded
lymphocytes were recovered
t cells were grown
lymphocytes were diminished
lymphocytes were identified
lymphocytes were labeled
lymphocytes were recorded
lymphocytes were stimulated
lymphocytes were counted
lymphocytes were encountered
lymphocytes were incubated
lymphocytes were washed
lymphocytes were distributed
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