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Exact(28)
Lymphocytes were washed with balanced salt solution three times.
The interface lymphocytes were washed twice with phosphate-buffered solution (PBS).
Lymphocytes were washed three times with, and resuspended in phosphate-buffered saline (PBS).
The lymphocytes were washed in fresh media before being left to adhere for 2 h in a 37°C incubator.
Non-adherent lymphocytes were washed off 24 hours later, and the adherent monocytes were fixed and stained.
Non-infiltrating or non-attaching donor lymphocytes were washed off before freezing of the skin explants and therefore would not contribute significantly to the results.
Similar(32)
Purified B-lymphocytes were washed and resuspended in HBSS and then incubated with 0.5 μM Fura-2, AM (acetoxymethyl ester) (Invitrogen, Carlsbad, CA, USA) at 106 cells/mL in fresh media at 37°C for 30 minutes.
The lymphocyte fractions were washed once by centrifugation at 440× g for 10 min at 4 °C and used for FCM.
Afterwards, the lymphocyte samples were washed with PBS, spun down to a pellet, and then stored at −80°C before DNA preparation.
Isolated PBMC, lymphocytes and neutrophils were washed, treated with protease inhibitors and lysed with an SDS solution containing 2-mercaptoethanol as described [26].
To examine DNA repair by the comet assay, lymphocytes after treatment with BisGMA were washed and resuspended in a fresh, RPMI 1640 medium preheated to 37°C.
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