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For in vitro stimulation, lymphocytes were stimulated with GP66 77 peptide (1 μg/ml) for 6 hr with Brefeldin A and 10 ng/ml IL-2 or 1 µg/ml PMA and ionomycin.
Quite opposite to our findings they observed the steepest ROC when lymphocytes were stimulated with HBHA while the stimulation with ESAT-6 produced the least discriminatory ROC.
To examine a role for CD152 in the contribution to the migratory properties of pro-inflammatory T lymphocytes upon primary stimulation, CD152−/− and CD152+/+ TCRtg CD4+CD62L+ T lymphocytes were stimulated with antigen under Th1 conditions and migration was evaluated in Transwell chemotaxis assays on day 5 (data not shown) or day 6 after stimulation (Fig. 1B); both assays gave similar results.
Lymphocytes were stimulated weekly and tested in cytotoxicity or cytokine release assays after three stimulations.
In this assay, isolated lymphocytes were stimulated by adding the phytohemagglutinin (PHA) in culture.
Lymphocytes were stimulated in vitro with PHA (a), tetanus antigen (b), and adenovirus antigen (c) as described.
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Our results show that it induces lymphocyte activation against B16F10 cells, primarily under conditions in which lymphocytes are stimulated by macrophages co-cultured (culture conditions Mϕ/Ly) in the presence of the CHM.
Neutrophils migrate in response to chemoattractant gradient and chemotactic factors while the migration of lymphocytes is stimulated by specific antigens, mitogens and other non-specific factors produced by lymphocytes [ 50].
The T-helper lymphocytes are stimulated by active macrophages in the LN and play an important role in the activation of B-lymphocytes and plasma cells to start the production of specific antibodies against antigens.
A recent publication by the Hotckhiss group showed that this could be achieved by exploiting the normal CD40 regulatory pathway through which lymphocytes are stimulated in antiapoptotic directions to produce clonal expansion and functional maturation (30 ).
It is well recognized that in the presence of IL-4 and co-stimulatory signals from Th2 cells, B lymphocytes are stimulated to switch to the production of IgE antibodies, while in the presence of IFNγ, the switch to the production of IgE is suppressed [ 27].
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