Sentence examples for insertion vector from inspiring English sources

Next, the GFP insertion vector with the appropriate stringency is chosen.

A modified dual-promoter insertion vector, pZCxz was constructed to target the genomic region of VTT, which corresponds to the Del III region of MVA [14].

For very poorly-folded proteins, one can start with a low-stringency insertion vector, such as 9/8_FR/SF or GFPi 9/8 SF/SF, to begin the directed evolution trajectory.

In other mutants, mitochondrial DNA was found co-integrated with insertion vector (data not show).

We also discovered 16 mutants with mitochondrial DNA co-integrated with the insertion vector.

This offset can be calculated as mean library fragment length minus insertion vector length.

The insertion vector-based gene targeting strategy used to generate this model has made it difficult to distinguish homozygous and heterozygous genotypes containing targeted Acadl alleles in LCAD-deficient mice.

The starting gene is cloned into the desired suite of GFP insertion vectors (typically the four 9/8 or 8/7 vectors) (Fig. 6A, Step 2.1).

One would expect that additional cycles of molecular evolution in GFP insertion vectors with increasing stringency would likely produce a more robust and soluble Rv2911 variant.

In contrast, fusions of the evolved Rv2911 to the high-stringency insertion vectors 8/7_FR/FR and 9/8_FR/FR are not fluorescent (Fig. 4B).

Next the panel of GFP insertion vectors containing the gene(s) is expressed at two temperatures, i.e. 27°C or 37°C, and the fluorescence evaluated (Fig. 6A, Step 2.2).